McCarter Biology

Biology IS Life

E.coli Insulin Factory

plasmid map

Learning Goals: construct a plasmid; determine the best restriction enzyme to use to cut the plasmid and DNA; choose the best antibiotic to determine if your recombined DNA was absorbed by the bacteria or not.

Key Terms:  plasmid, recombinant DNA, restriction enzymes, transformation, antibiotic resistance, restriction site, plasmid map.

download a copy of the E.coli Insulin Factory lab


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