Pre-lab: HHMI tutorial of 8 slides plus a quick video clip about the gene for p53 tumor supressor protein (the “Guardian of the Genome“) and how it is related to cancer.
1. Develop a pedigree to show how cancer is inherited in one family.
2. Run a gel electrophoresis to separate the TP53 genes of a woman with breast cancer.
3. Determine the patient’s genotype based on the gel electrophoresis results.
Key Concepts: pedigrees, gel electrophoresis, restriction enzymes, cancer, TP53 gene and mutations.
*this is an EDVOTEK lab #115.